Descripton
C48H78O19;959.15

【HPLC】

1、Check sample Asiaticoside and Madecassoside (C=0.8mg/ml methanol)  

2、Product sample Gotu kola P.E. 10% (C=1.2mg/ml methanol)

3、Instrument Waters 515

4、detector Waters 2487

5、Wavelength: UV 204nm

6、Chromatographic column:ODS-C18  4.6×250mm

7、Mobile phase： Acetonitrile ：Methanol ：Water＝22 28 50

8、Rate 0.8ml/Min

Pharmacological action

Helps prevent Varicose Veins/Hemorrhoids :The circulatory improvement occurs
  because gotu kola decreases vein hardening, improves the connective tissue around
  veins, and helps the blood to flow throug

Overcoming stress and fatigue: Gotu kola contains contains sedative and an 
  abundance of  "anti-stress" B vitamins, this kind of vitamins can be effect in
  relaxing body. It can be used as a nervine and general tonic.

Wound-healing and scars:  Research showed that a Gotu kola extract enriched
  with asiaticoside stimulated the synthesis of collagen by fibroblasts in vitro and it
  helps reduce the redness and thickness of scars by causing them to mature faster. 
  So Gotu kola extract can be used for wound healing purpose and removal of scars
  in the skin .

Leprosy ：Research has shown that asiaticoside may provide treatment for
  leprosy.Leprosy-causing bacteria are coated in a wax-like substance that the
  immune system can't penetrate. However, gota kola dissolves the waxy coating,
  allowing the immune system to destroy the bacteria.

Mental disorder: Alcoholic extract prolonged the hypnotic effect of Sodium
  phenobarbitone in mice and rats1. The centella glycosides when injected into
  anaesthetised rats significantly lowered the blood pressure2. It was observed that
  alcoholic extract decreased the acetylcholine and histamine content of the whole
  brain homogenate of albino rats. Inhibition of the increase in acetylcholine in the
  brain in stressed rats was observed3.

1.Agarwal, S.S. : J.Res. Ayur. Siddha, 11:11 (1981).

2.Ramaswamy, A.S. et al. : J.Res. Ind. Med., 4:2 (1970).

3.Singh RH et al., J. Res. Ind. Med. Homeo, 14:3 (1979)

Clinical ApplicationsTraditionally used for The decoction of the whole herb is used for treatment of colds, sunstroke, tonsillitis,
urinary tract infection, hepatitis, jaundice, skin diseases, chronic rheumatism, amenorrhoea
and dysentery and also a blood purifier. It is also used as a folk remedy for leprosy,
lumps and syphilis, tuberculosis and for improving mental function1-2.

Chinese medicine uses various parts of the plant. The leaves are used for leukorrhea and
fevers that are toxic, while other types of fevers and boils are treated with gotu kola
shoots. Gotu kola has been used for centuries as a brain tonic and used for longevity has
become very popular in China. .

Traditional use of Gotu Kola in India and Indonesia included wound treatment. In the
1800s, it became part of Indian medicine practice and was used to treat many skin
conditions including leprosy, varicose ulcers, and eczema, as well as fever, diarrhea, and
absence of menses.

In Africa peoples used for the treatment of leprosy (the asiaticoside content dissolves the
waxy coating of the leprosy bacteria - allowing the immune system to destroy the
bacteria), bronchitis, asthma, syphilis and wound healing

Can be used for improving memory, overcoming stress and fatigue.3

Increases intelligence quotient in mentally retarded children.
Helps prevent Varicose Veins/Hemorrhoids

Studies have also shown that gotu kola has positive effects on varicose veins and
hemorrhoids.A 1992 review of all the gotu kola studies available concluded that gotu kola
extract provides a dose-related improvement in venous insufficiency symptoms, reducing
foot swelling, ankle edema, and fluid leakage from the veins.4

Helps heal Wounds / Burns / Scars/ Skin Inflammation
Gotu kola has positive effects on various skin problems. Animal research has shown
that tripenoid asiaticoside may help wounds heal quicker. Other studies showed that gotu
kola helped in healing surgical wounds of the ear, nose, and throat, and promoted healing
of episiotomies, gangrene skin grafts, and some skin ulcers.

In a French study done in 1966 it was found that it had a significant healing effect when
used after episiotomy - a surgical cut of the vulva to prevent tearing during childbirth. The
cut healed more rapidly than with standard treatment.

Gotu kola was used in treatment of patients with second or third degree burns from
boiling water or gas explosions if the burn is treated immediately. The extract can stop or
reduce the shrinkage and swelling of the skin caused by infection and inhibited scar
formation, increasing healing and reducing fibrosis.

It has been used to prevent the development of keloid (bulging, enlarged) scars following
surgery,studies suggest that gotu kola extracts may be useful in treating anal
fissures,bladder ulcers, dermatitis, , perineal lesions,striae gravidarum

Clinical trials also show that gotu kola's tripenoids, when purified, can lessen the ravages
of scleroderma. In addition to decreased skin hardening, patients have noticed a lessening
of joint pain and improved finger motility..

Treatment of liver and kidney problems
It has been used for centuries in the treatment of liver and kidney problems, and has
once again become popular as an alternative treatment for people suffering from
hepatitis as well as alcoholic liver disease.

Research shows gotu kola extract also can be effective in other diseases,

anti-spasmodic activity, hypothyroidism,venous hypertension,antidote to cholera,gastric catarrh
urethritis,periodontal disease,reduce cellulite,liver cirrhosis,tumors,epilepsy,alopecia,psoriasis
jaundice,anemia,diabetes,phlebitis,tonsillitis,insomnia.

References:1. Kartnig. T. in "Herbs, Spices and Medicinal Plants- Recent Advances in Botany, Horticulture and Pharmacology", eds. L.E. Craker and J. E. Simons, Oryx Press Phoenix, 1988; 3 : 145.
2. M. Badruzzman Siddiqui and W. Hussain, Fitoterapia, 1992; 63(3) : 245.
3. Murray MT. Centella asiatica. American Journal of Natural Medicine. Vol. 3. No. 6. July. 1996
4. Cesarone MR, et al. Activity of Centella asiatica in venous insufficiency. Minerva Cardioangiol 42: 137–143, 1992.