Cactus Botanics
Cactus Botanics
Issue 8 · 2014
Pure Chondroitin Sulfate from Cactus Botanics
China Office:Cactus Botanics Limited

Add:6A&B,Block 2,No 628,Zhang Yang Road,
Pudong New District,Shanghai,China,200122

P h:86-21-5169.6902

Fax:86-21-5106.2932

USA Office:GB Biotech Inc.

Add:1108 W Valley Blvd#6278,
Alhambra,CA 91803

P h:562.219.2167

Fax:562.546.1323

Email:info@cactusbotanics.com

When it comes to nourishing aging joints, chondroitin sulfate is the most recognized supplement consumers seek, along with glucosamine. Because awareness and trust remain so high, it is incumbent upon brand marketers to ensure that your chondroitin source is safe and proven pure. Unfortunately, there have been some suppliers that adulterate their chondroitin, and you may be none the wiser.

The adulteration discovered recently is Sodium Hexametaphosphate, which is normally used as an industrial chemical and is easily available at a very low price. Although is often used effectively -- and appropriately -- in detergent or water treatment agents, it is unsafe as a food additive for human ingestion.

How does Cactus Botanics Ensure
Chondroitin Sulfate Purity?

Chondroitin sulfate is typically derived from animal cartilages, such as chicken, bovine, shark, etc. Our experts employ four test methods to determine the quality of our chondroitin sulfate material and its purity.

HPLC
High Performance Liquid Chromatography, is a qualitative test method. Chondroitin sulfate is dispersed evenly by acetonitrile and dissolved in water. After the liquid chromatography separation, 192 mm wavelength is in the determination of external standard method.
CPC
CPC is a test method for purity. This method is based on the formation of turbidity reactions with organic anions such as sulfate or carboxylate ions under slightly basic conditions. The key is organic anion. Further, the principle behind this method is the binding of the cationic CPC molecules with the negatively charged chondroitin sulphate to form insoluble ion pairs seen as a precipitate. The absorbance of the solution is monitored and by comparing with a chondroitin standard solution of known concentration, the amount of chondroitin in the test solution can be determined.
EHPLC

There is also another test method introduced to the market, known as EHPLC. It is a method based on special enzymatic hydrolysis to produce disaccharides Di-4S, Di-6S and Di-0S that make up the chondroitin sulfate A and C and non-sulfated chondroitin. The resulting disaccharides are then measured by HPLC with a UV detector at 240 nm.

Note: As this method only can be executed by a few laboratories worldwide, and only one in the US ,the details are not publicly available yet. When using this testing method, there is some trouble in the coherence and consistency of lab results. Owing to the confidentiality and non-impartiality, EHPLC is hard to be a standard test method to the public.

Specific
Rotation
In chemistry, specific rotation ([α]) is a property of a chiral chemical compound. The variance of specific rotation with wavelength — a phenomenon known as optical rotatory dispersion — can be used to find the absolute configuration of a molecule. The specific rotation of chondroitin sulfate is -20°~-30°.
Why buy from Cactus Botanics?
Local stock that reaches the standard of 90% tested by CPC, available in Los Angeles.The origin is China, known for its superior purity and quality of chondroitin sulfate.
We may supple you with a variety of chondroitin sulfate materials in the standard of 90% tested by HPLC from China.
Our customer service is trained to answer your questions, and to ensure satisfaction with follow-up communications.
For Further Study
  1. David JI, Mark Roman, Joseph Zhou, Determination of Chondroitin Sulfate Content in Raw Materials and Dietary Supplement by High-Performance Liquid Chromatography with Ultraviolet Detection After Enzymatic Hydrolysis: Single-Laboratory Validation, J AOAC Int. 2007; 90(3); 659-669.
  2. W. Garnjanagoonchorn, L. Wongekalak, A. Engkagul, Determination of Chondroitin Sulfate from differ ent sources of cartilage, Chemical Engineering and Processing: Process Intensification,2007; 46(5); 465-471
  3. Daiki Kubomura, Ayano Masui, Koichi Okada, A practical method for determining chondroitin sulfate content in shark cartilage materials and products, Jpn. J. Food Chem. Safety, 2012; 19(3)
  4. Chong-Tai Kim, Naiyana Gujral, Advaita Ganguly, Chondroitin sulphate extracted from antler cartilage using high hydrostatic pressure and enzymatic hydrolysis, Biotechnology Reports, 2014; 4; 14-20
  5. David A. Keire, Michael L. Trehy, John C. Reepmeyer, Analysis of crude heparin by 1H NMR, capillary electrophoresis, and strong-anion-exchange-HPLC for contamination by over sulfated chondroitin sulfate, Journal of Pharmaceutical and Biomedical Analysis, 2010; 51(4): 921-926
  6. I Koshiishi, M Takenouchi, T Hasegawa, Enzymatic Method for the Simultaneous Determination of Hyaluronan and Chondroitin Sulfates Using High-Performance Liquid Chromatography, Analytical Biochemistry, 1998; 265(1):6
  7. K Murata, Yokoyama, A high-performance liquid chromatography for constituent disaccharides of chon droitin sulfate and dermatan sulfate isomers, Analytical Biochemistry, 1985; 146(2): 327
  8. Arvan L Fluharty, Jeffrey A Glick, Nicholas Matusewicz, High-performance liquid chromatography deter mination of unsaturated disaccharides produced from chondroitin sulfates by chondroitinases, Biochemical Medicine, 1982; 27(3): 352
We look forward to always better serve our esteemed customers.
For mor information:info@cactusbotanics.com
October 8-9 , 2014 ▪ Las Vegas
See us in booth#30108

5-7 May 2015

Palexpo,Geneva,Switzerland

See us in booth # M107
June 24-26 2015
Shanghai New International Expo Center. Shanghai. CHINA
See us in booth #E1 A25
READ MORE
We do hope to better serve our esteemed customers with our Finest Quality Products.
We will submit Issues 15th per month.

Click below the links for the issues of previous years:
2013 Issues package
2012 Issues package
2011 Issues package